Specification of DNA Binding Activity of NF- B Proteins

comparison of catalytic activity of heteropoly compounds in the synthesis of bis(indolyl)alkanes.

heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...

Synthesis, Characterization, DNA Binding and Nuclease Activity of Cobalt(II) Complexes of Isonicotinoyl Hydrazones

Cobalt(II)  complexes of isonicotinoyl hydrazones of two series of ligands have been synthesized and characterized on the basis of elemental analyses, molar conductance, magnetic moment, mass, IR, UV spectral data. Electrochemical behavior of ligands and complexes has been investigated by using cyclic voltammetry. Cyclic voltammetric studies reveal that the oxidation/reduct...

Control of nuclear factor-kappa B DNA-binding activity by inhibitory proteins containing ankyrin repeats.

Analysis of epidermal proteins for DNA-binding activity.

A group of DNA-binding proteins from the soluble extract of newborn rat epidermis have been separated by chromatography using DNA-cellulose columns. The electrophoretogram of the DNA-binding proteins eluted from a single stranded DNA-cellulose column shows five major proteins of molecular weights ranging between 25K to 40K. Both the epidermal protein filaggrin and most keratins, except two high...

Extraction of nuclear proteins with increased DNA binding activity.

We have developed a method for the rapid extraction of nuclear proteins from cultured cells. The ammonium sulfate method described here extracts larger quantities of proteins that retain DNA binding activity than the modified Dignam method and another popular method used for the extraction of transcription factors. The ammonium sulfate method is rapid and can be used to process a large number o...